Hotspot

matK

Genome · 1441-2934 · 1494 bp

Back to Pteridium

Pi 0.00602

MarkerSeek score 55.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: Genome
length_bp: 1494
variable_sites: 9
pi: 0.00602
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCTCTTTGCAAGGAGATCAA	AATGCTTGGGAGTTACTGCA	40.0	45.0	54.3	57.4	1534 bp	1.0	46.0
matK_p2	TTCTCTTTGCAAGGAGATCA	AATGCTTGGGAGTTACTGCA	40.0	45.0	54.3	57.4	1535 bp	1.0	46.0
matK_p3	TTCTCTTTGCAAGGAGATCAA	AATGCTTGGGAGTTACTGCA	38.1	45.0	55.1	57.4	1535 bp	1.0	44.9
matK_p4	TTTCTCTTTGCAAGGAGATCA	AATGCTTGGGAGTTACTGCA	38.1	45.0	55.1	57.4	1536 bp	1.0	44.9
matK_p5	TCTCTTTGCAAGGAGATCAA	CAATGCTTGGGAGTTACTGC	40.0	50.0	54.3	57.1	1535 bp	1.0	43.2