Hotspot

infA

Genome · 77591-77833 · 243 bp

Back to Pteridium

Pi 0.00823
MarkerSeek score 45.70
Primer available yes
Label infA

Feature Metadata

label_name
infA
feature_type
gene
region
Genome
length_bp
243
variable_sites
2
pi
0.00823
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA_p1 GGTAGTGACACCAGCAACAA TCTGTGAATAATCTGGGAGGT 50.0 42.9 58.0 55.8 303 bp 1.0 54.3
infA_p2 AGTGACACCAGCAACAAAGT TCTGTGAATAATCTGGGAGGT 45.0 42.9 57.9 55.8 300 bp 1.0 53.5
infA_p3 GGTAGTGACACCAGCAACAA TCTGTGAATAATCTGGGAGGTT 50.0 40.9 58.0 56.5 303 bp 1.0 52.4
infA_p4 ACTAGGTAGTGACACCAGCA TCTGTGAATAATCTGGGAGGT 50.0 42.9 57.7 55.8 307 bp 1.0 52.1
infA_p5 AGTGACACCAGCAACAAAGT TCTGTGAATAATCTGGGAGGTT 45.0 40.9 57.9 56.5 300 bp 1.0 51.7