Hotspot

ccsA

Genome · 112124-113074 · 951 bp

Back to Pteridium

Pi 0.00526
MarkerSeek score 61.80
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
Genome
length_bp
951
variable_sites
5
pi
0.00526
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TCGAGATCGATCAACCAAACT AAACCGAAAGCCTTTGTTGC 42.9 45.0 57.1 58.1 1016 bp 1.0 64.9
ccsA_p2 TCCCCTTATTCCCTTTCGTT AAACCGAAAGCCTTTGTTGC 45.0 45.0 56.1 58.1 1080 bp 1.0 64.8
ccsA_p3 TCGAGATCGATCAACCAAACT GACTAACCCCTGCTTTGGTT 42.9 50.0 57.1 57.7 1044 bp 1.0 63.1
ccsA_p4 TCCCCTTATTCCCTTTCGTTT AAACCGAAAGCCTTTGTTGC 42.9 45.0 56.9 58.1 1080 bp 1.0 63.0
ccsA_p5 TCCCCTTATTCCCTTTCGTT GACTAACCCCTGCTTTGGTT 45.0 50.0 56.1 57.7 1108 bp 1.0 63.0