Hotspot

ndhC-trnV-UAC

Genome · 48299-50622 · 2324 bp

Back to Pteridium

Pi 0.00646

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 2324
variable_sites: 15
pi: 0.00646
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCTTTTCGAGTAGGGGCAAT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.6	57.9	2456-2476 bp	1.0	76.6
ndhC-trnV-UAC_p2	CCTTTTCGAGTAGGGGCAAT	CACAAAAGTGGCATGTTGGG	50.0	50.0	57.6	58.1	2538-2558 bp	1.0	76.1
ndhC-trnV-UAC_p3	CCTTTTCGAGTAGGGGCAAT	CCACAAAAGTGGCATGTTGG	50.0	50.0	57.6	58.1	2539-2559 bp	1.0	76.1
ndhC-trnV-UAC_p4	CCTTTTCGAGTAGGGGCAAT	CGATCTGCATGACCGAGAAT	50.0	50.0	57.6	57.9	2482-2502 bp	1.0	76.0
ndhC-trnV-UAC_p5	CCTTTTCGAGTAGGGGCAAT	CCGAGAATGTCTACGGTTCG	50.0	55.0	57.6	58.2	2470-2490 bp	1.0	75.7