Hotspot

psbM-petN

Genome · 28257-29622 · 1366 bp

Back to Pteridium

Pi 0.00587

MarkerSeek score 62.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 1366
variable_sites: 8
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GCCACCGCACTTTTTATTCC	CCCCGACGGTGATTTTTACA	50.0	50.0	58.0	58.2	1504-1506 bp	1.0	79.0
psbM-petN_p2	ATATTCTCGCGTTCGTAGCC	CCCCGACGGTGATTTTTACA	50.0	50.0	58.0	58.2	1521-1523 bp	1.0	78.7
psbM-petN_p3	GCCACCGCACTTTTTATTCC	GTAGTTCGGGGAAGAAGTGG	50.0	55.0	58.0	57.6	1469-1471 bp	1.0	77.5
psbM-petN_p4	CGGCCGCTCAGAATAACTAG	CCCCGACGGTGATTTTTACA	55.0	50.0	58.2	58.2	1446-1448 bp	1.0	77.3
psbM-petN_p5	ATATTCTCGCGTTCGTAGCC	GTAGTTCGGGGAAGAAGTGG	50.0	55.0	58.0	57.6	1486-1488 bp	1.0	77.2