Hotspot

psbK-psbI

Genome · 7205-7710 · 506 bp

Back to Pteridium

Pi 0.00792

MarkerSeek score 61.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 506
variable_sites: 4
pi: 0.00792
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TGCCAAATTACCCGAAGCTT	GGATTTCGTCCGGGATCATT	45.0	50.0	58.1	57.7	718-719 bp	1.0	77.9
psbK-psbI_p2	TTGCCAAATTACCCGAAGCT	GGATTTCGTCCGGGATCATT	45.0	50.0	58.1	57.7	719-720 bp	1.0	77.8
psbK-psbI_p3	ACCCGAAGCTTATGCCATTT	GGATTTCGTCCGGGATCATT	45.0	50.0	57.9	57.7	709-710 bp	1.0	77.4
psbK-psbI_p4	TAGCTTTTGTTTGGCAAGCC	GGATTTCGTCCGGGATCATT	45.0	50.0	57.5	57.7	638-639 bp	1.0	74.4
psbK-psbI_p5	TGCCAAATTACCCGAAGCTT	TTTCGTCCGGGATCATTTGA	45.0	45.0	58.1	57.2	715-716 bp	1.0	73.7