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psaJ-rps18

LSC · 67747-68527 · 781 bp

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Pi 0.0439

MarkerSeek score 74.10

Primer available yes

Label psaJ-rps18

Feature Metadata

label_name: psaJ-rps18
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 34
pi: 0.0439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rps18_p1	ATGCGTTGACATTCCCCTTT	CAATTCGATCCCCCGATTGA	45.0	50.0	58.1	57.7	895-912 bp	1.0	80.9
psaJ-rps18_p2	ATGCGTTGACATTCCCCTTT	TCAATTCGATCCCCCGATTG	45.0	50.0	58.1	57.7	896-913 bp	1.0	80.9
psaJ-rps18_p3	TGCGTTGACATTCCCCTTTT	CAATTCGATCCCCCGATTGA	45.0	50.0	58.3	57.7	894-911 bp	1.0	79.1
psaJ-rps18_p4	TGCGTTGACATTCCCCTTTT	TCAATTCGATCCCCCGATTG	45.0	50.0	58.3	57.7	895-912 bp	1.0	79.1
psaJ-rps18_p5	ATGCGTTGACATTCCCCTTT	TTCGATCCCCCGATTGAATC	45.0	50.0	58.1	57.5	892-909 bp	1.0	78.6