Hotspot

accD-psaI

LSC · 59042-59822 · 781 bp

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Pi 0.0279

MarkerSeek score 69.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 20
pi: 0.0279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCGTTTCATGCTTTTCTCTCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.1	58.0	839-899 bp	1.0	76.5
accD-psaI_p2	GCGTTTCATGCTTTTCTCTCC	AAGAAGCCATTGCAATTGCC	47.6	45.0	58.1	57.9	845-905 bp	1.0	75.9
accD-psaI_p3	TGCGTTTCATGCTTTTCTCTC	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.5	58.0	840-900 bp	1.0	73.3
accD-psaI_p4	GCGTTTCATGCTTTTCTCTCC	GCCGGAAATACTAGGCCTAC	47.6	55.0	58.1	57.5	828-888 bp	1.0	73.1
accD-psaI_p5	TGCGTTTCATGCTTTTCTCTC	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.5	57.9	846-906 bp	1.0	72.7