Hotspot

trnV-UAC-trnM-CAU

LSC · 52729-52968 · 240 bp

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Pi 0.0468

MarkerSeek score 74.40

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 11
pi: 0.0468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	ACCAACTGAGCTATAGCCCT	TTGAACCAATGACTCTCGCC	50.0	50.0	57.8	58.2	315-318 bp	1.0	81.1
trnV-UAC-trnM-CAU_p2	ACCTTGAAAAACATTGGCGC	TTGAACCAATGACTCTCGCC	45.0	50.0	58.1	58.2	355-358 bp	1.0	81.0
trnV-UAC-trnM-CAU_p3	CCTTGAAAAACATTGGCGCA	TTGAACCAATGACTCTCGCC	45.0	50.0	58.1	58.2	354-357 bp	1.0	81.0
trnV-UAC-trnM-CAU_p4	ACCTTGAAAAACATTGGCGC	TCTCGCCGTATGAAAGCAAT	45.0	45.0	58.1	57.7	342-345 bp	1.0	80.2
trnV-UAC-trnM-CAU_p5	CCTTGAAAAACATTGGCGCA	TCTCGCCGTATGAAAGCAAT	45.0	45.0	58.1	57.7	341-344 bp	1.0	80.2