Hotspot

trnF-GAA-ndhJ

LSC · 47865-49929 · 2065 bp

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Pi 0.0655

MarkerSeek score 75.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 2065
variable_sites: 134
pi: 0.0655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CGTTCCCAATGTGCCTATGA	55.0	50.0	58.0	58.0	2459 bp	0.5	59.7
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	ATCTACGTTCCCAATGTGCC	55.0	50.0	58.0	58.0	2464 bp	0.5	59.7
trnF-GAA-ndhJ_p3	AATTGACACAGCCCCCATTT	CGTTCCCAATGTGCCTATGA	45.0	50.0	58.0	58.0	2514 bp	0.5	59.5
trnF-GAA-ndhJ_p4	ATTGACACAGCCCCCATTTT	CGTTCCCAATGTGCCTATGA	45.0	50.0	58.0	58.0	2513 bp	0.5	59.5
trnF-GAA-ndhJ_p5	ATTGACACAGCCCCCATTTT	ATCTACGTTCCCAATGTGCC	45.0	50.0	58.0	58.0	2518 bp	0.5	59.5