Hotspot

rpoB-trnC-GCA

LSC · 26357-27435 · 1079 bp

Back to Psychotria

Pi 0.0356

MarkerSeek score 74.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1079
variable_sites: 37
pi: 0.0356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCCGTCAAACCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1212-1244 bp	1.0	82.3
rpoB-trnC-GCA_p2	AGTTCTTCCGTCAAACCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1213-1245 bp	1.0	82.2
rpoB-trnC-GCA_p3	CTTCCGTCAAACCCTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1209-1241 bp	1.0	80.5
rpoB-trnC-GCA_p4	GTTCTTCCGTCAAACCCTGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1194-1226 bp	1.0	80.2
rpoB-trnC-GCA_p5	AGTTCTTCCGTCAAACCCTG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1195-1227 bp	1.0	80.1