Hotspot

trnS-GCU-trnS-CGA

LSC · 8357-9151 · 795 bp

Back to Psychotria

Pi 0.0556

MarkerSeek score 78.80

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 41
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	AGTCCACTCAGCCATCTCTC	ACCACTAAACTATACCCGCC	55.0	50.0	58.5	56.7	782-836 bp	1.0	73.6
trnS-GCU-trnS-CGA_p2	TAGTCCACTCAGCCATCTCT	ACCACTAAACTATACCCGCC	50.0	50.0	57.2	56.7	783-837 bp	1.0	71.1
trnS-GCU-trnS-CGA_p3	TCCACTCAGCCATCTCTCC	ACCACTAAACTATACCCGCC	57.9	50.0	58.1	56.7	780-834 bp	1.0	69.0
trnS-GCU-trnS-CGA_p4	TTAGTCCACTCAGCCATCTCT	ACCACTAAACTATACCCGCC	47.6	50.0	57.9	56.7	784-838 bp	1.0	68.9
trnS-GCU-trnS-CGA_p5	TAGTCCACTCAGCCATCTCTC	ACCACTAAACTATACCCGCC	52.4	50.0	58.3	56.7	783-837 bp	1.0	67.0