Hotspot

rps16-trnQ-UUG

LSC · 6287-7235 · 949 bp

Back to Psychotria

Pi 0.0368
MarkerSeek score 73.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
949
variable_sites
34
pi
0.0368
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1022-1034 bp 1.0 82.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGATCTAAATGGGGCGTGG 45.0 55.0 58.1 58.1 1080-1092 bp 1.0 82.4
rps16-trnQ-UUG_p3 CACAATGGATCGGGACCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1042-1054 bp 1.0 81.3
rps16-trnQ-UUG_p4 CACAATGGATCGGGACCTTC GAGATCTAAATGGGGCGTGG 55.0 55.0 58.3 58.1 1100-1112 bp 1.0 80.8
rps16-trnQ-UUG_p5 ATGGATCGGGACCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1038-1050 bp 1.0 80.7