Hotspot

trnH-GUG-psbA

LSC · 72-399 · 328 bp

Back to Psychotria

Pi 0.0588

MarkerSeek score 72.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 18
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	780-814 bp	1.0	83.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GTTATACAACGGCGGTCCTT	55.0	50.0	58.1	58.0	1097-1131 bp	1.0	82.2
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GTTATACAACGGCGGTCCTT	50.0	50.0	57.9	58.0	1111-1145 bp	1.0	82.2
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GTTATACAACGGCGGTCCTT	50.0	50.0	57.9	58.0	1110-1144 bp	1.0	82.1
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GTTATACAACGGCGGTCCTT	50.0	50.0	57.9	58.0	1112-1146 bp	1.0	82.1