Hotspot

trnV-GAC-rps12

IRa · 107406-108982 · 1577 bp

Back to Psittacanthus

Pi 0.0893

MarkerSeek score 80.70

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1577
variable_sites: 230
pi: 0.0893
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	AGGTGACACTCTACCACTGA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.3	57.7	741-1719 bp	1.0	84.5
trnV-GAC-rps12_p2	GGTGACACTCTACCACTGAG	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.0	57.7	740-1718 bp	1.0	81.8
trnV-GAC-rps12_p3	GTCAAGGTGACACTCTACCAC	TGGCAACTTTACGTAAGGCA	52.4	45.0	58.0	57.7	745-1723 bp	1.0	81.7
trnV-GAC-rps12_p4	CAAGGTGACACTCTACCACTG	TGGCAACTTTACGTAAGGCA	52.4	45.0	58.0	57.7	743-1721 bp	1.0	81.7
trnV-GAC-rps12_p5	AAGGTGACACTCTACCACTGA	TGGCAACTTTACGTAAGGCA	47.6	45.0	58.0	57.7	742-1720 bp	1.0	81.6