Hotspot

ccsA-psaC

SSC · 94095-94640 · 546 bp

Back to Psittacanthus

Pi 0.121

MarkerSeek score 78.20

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 546
variable_sites: 116
pi: 0.121
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	GGGAGCTGTATGGGCTAATG	CATGTATCGGGTGCACTCAA	55.0	50.0	57.8	58.0	831-1181 bp	1.0	90.3
ccsA-psaC_p2	GGGAGCTGTATGGGCTAATG	TGTATCGGGTGCACTCAATG	55.0	50.0	57.8	58.0	829-1179 bp	1.0	90.3
ccsA-psaC_p3	GGGAGCTGTATGGGCTAATG	GTATCGGGTGCACTCAATGT	55.0	50.0	57.8	58.0	828-1178 bp	1.0	90.3
ccsA-psaC_p4	CGCTCTTCAATCCCAATGGT	CATGTATCGGGTGCACTCAA	50.0	50.0	58.2	58.0	1164-1514 bp	1.0	89.9
ccsA-psaC_p5	CGCTCTTCAATCCCAATGGT	TGTATCGGGTGCACTCAATG	50.0	50.0	58.2	58.0	1162-1512 bp	1.0	89.9