Hotspot

rps12-trnV-GAC

IRb · 84579-86155 · 1577 bp

Back to Psittacanthus

Pi 0.0792
MarkerSeek score 80.20
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1577
variable_sites
208
pi
0.0792
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.86
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA AGGTGACACTCTACCACTGA 45.0 50.0 57.7 57.3 741-1719 bp 1.0 84.5
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GGTGACACTCTACCACTGAG 45.0 55.0 57.7 57.0 740-1718 bp 1.0 81.8
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA GTCAAGGTGACACTCTACCAC 45.0 52.4 57.7 58.0 745-1723 bp 1.0 81.7
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA CAAGGTGACACTCTACCACTG 45.0 52.4 57.7 58.0 743-1721 bp 1.0 81.7
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA AAGGTGACACTCTACCACTGA 45.0 47.6 57.7 58.0 742-1720 bp 1.0 81.6