Hotspot

petA-psbJ

LSC · 52257-52778 · 522 bp

Back to Psittacanthus

Pi 0.0466

MarkerSeek score 81.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 51
pi: 0.0466
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TATGTAGGCGGGAATAGGGG	ACACAATCAAACCCGAACGA	55.0	45.0	58.1	58.0	1308-1362 bp	1.0	87.4
petA-psbJ_p2	TATGTAGGCGGGAATAGGGG	ACGACACAATCAAACCCGAA	55.0	45.0	58.1	58.0	1311-1365 bp	1.0	87.4
petA-psbJ_p3	TATGTAGGCGGGAATAGGGG	ACGGACTAGCTGTACCTACC	55.0	55.0	58.1	58.0	1401-1455 bp	1.0	87.0
petA-psbJ_p4	TATGTAGGCGGGAATAGGGG	GGACTAGCTGTACCTACCGT	55.0	55.0	58.1	58.0	1399-1453 bp	1.0	87.0
petA-psbJ_p5	TATGTAGGCGGGAATAGGGG	GAGCCGACAAGGAATTCCAT	55.0	50.0	58.1	58.0	1546-1595 bp	1.0	86.5