Hotspot

psaA-ycf3

LSC · 36044-36653 · 610 bp

Back to Psittacanthus

Pi 0.045

MarkerSeek score 82.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 56
pi: 0.045
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTGGCCCATTCTTCGAAAGA	TGGTTGAAGATCACGAGACG	50.0	50.0	57.9	57.7	717-798 bp	1.0	86.4
psaA-ycf3_p2	TCTGGCCCATTCTTCGAAAG	TGGTTGAAGATCACGAGACG	50.0	50.0	57.9	57.7	718-799 bp	1.0	86.3
psaA-ycf3_p3	CTGGCCCATTCTTCGAAAGA	GGTTGAAGATCACGAGACGT	50.0	50.0	57.9	57.7	716-797 bp	1.0	86.3
psaA-ycf3_p4	TCTGGCCCATTCTTCGAAAG	GGTTGAAGATCACGAGACGT	50.0	50.0	57.9	57.7	717-798 bp	1.0	86.3
psaA-ycf3_p5	GTCTGGCCCATTCTTCGAAA	TGGTTGAAGATCACGAGACG	50.0	50.0	58.2	57.7	719-800 bp	1.0	85.8