Hotspot

trnT-GGU-psbD

LSC · 26803-27370 · 568 bp

Back to Psittacanthus

Pi 0.0759

MarkerSeek score 83.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 80
pi: 0.0759
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGGATTTGAACCAGCGTAGA	TCAGGGCCCCATAGTAACAA	50.0	50.0	58.0	58.0	1543-1975 bp	1.0	89.8
trnT-GGU-psbD_p2	CGGATTTGAACCAGCGTAGA	TTCAGGGCCCCATAGTAACA	50.0	50.0	58.0	58.0	1544-1976 bp	1.0	89.8
trnT-GGU-psbD_p3	CGGATTTGAACCAGCGTAGA	AGCTTGGGTGGGGTTAAAAG	50.0	50.0	58.0	58.0	1976-2408 bp	1.0	89.2
trnT-GGU-psbD_p4	CGGATTTGAACCAGCGTAGA	AGTTTCTTCAGCTTGGGTGG	50.0	50.0	58.0	58.0	1985-2417 bp	1.0	89.1
trnT-GGU-psbD_p5	CGACCCAGAAGGAATCCTTG	AGCTTGGGTGGGGTTAAAAG	55.0	50.0	58.0	58.0	1908-2262 bp	0.6	68.6