Hotspot

petN-psbM

LSC · 23860-24647 · 788 bp

Back to Psittacanthus

Pi 0.057

MarkerSeek score 83.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 93
pi: 0.057
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTATCATTTTGGCGGCATGG	TCCACCCCGAACTTTTGAAA	50.0	45.0	57.9	57.6	1994-2066 bp	0.4	59.0
petN-psbM_p2	AGGGGGAGGGTTCAAGATAC	TCCACCCCGAACTTTTGAAA	55.0	45.0	58.1	57.6	2082 bp	0.2	43.6
petN-psbM_p3	AGGGGGAGGGTTCAAGATAC	TTCCACCCCGAACTTTTGAA	55.0	45.0	58.1	57.6	2083 bp	0.2	43.6
petN-psbM_p4	GGGGCCGGGCTATAACTATA	TCCACCCCGAACTTTTGAAA	55.0	45.0	58.1	57.6	1764 bp	0.2	43.5
petN-psbM_p5	GGGGCCGGGCTATAACTATA	TTCCACCCCGAACTTTTGAA	55.0	45.0	58.1	57.6	1765 bp	0.2	43.5