Hotspot

trnK-UUU-trnQ-UUG

LSC · 3789-4448 · 660 bp

Back to Psittacanthus

Pi 0.105

MarkerSeek score 70.50

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 70
pi: 0.105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.34
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	ACGAGTACTCTACCGTCGAG	AGACAGGTATGACCGGCATA	55.0	50.0	58.1	57.9	1227 bp	0.2	46.7
trnK-UUU-trnQ-UUG_p2	AGACGAGTACTCTACCGTCG	AGACAGGTATGACCGGCATA	55.0	50.0	58.1	57.9	1229 bp	0.2	46.7
trnK-UUU-trnQ-UUG_p3	CGAGTACTCTACCGTCGAGT	AGACAGGTATGACCGGCATA	55.0	50.0	58.1	57.9	1226 bp	0.2	46.7
trnK-UUU-trnQ-UUG_p4	GACGAGTACTCTACCGTCGA	AGACAGGTATGACCGGCATA	55.0	50.0	58.1	57.9	1228 bp	0.2	46.7
trnK-UUU-trnQ-UUG_p5	CGAGTACTCTACCGTCGAGT	GCTATTCGGAGGTTCGAGTC	55.0	55.0	58.1	57.9	721 bp	0.2	46.4