primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	ACGAGTACTCTACCGTCGAG	AGACAGGTATGACCGGCATA	20	20	55.0	50.0	58.1	57.9	20.3	0.0	0.162222	3789	4448	1227-1227	0.200000	46.7
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	AGACGAGTACTCTACCGTCG	AGACAGGTATGACCGGCATA	20	20	55.0	50.0	58.1	57.9	21.8	0.0	0.162222	3789	4448	1229-1229	0.200000	46.7
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	CGAGTACTCTACCGTCGAGT	AGACAGGTATGACCGGCATA	20	20	55.0	50.0	58.1	57.9	20.3	0.0	0.162222	3789	4448	1226-1226	0.200000	46.7
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	GACGAGTACTCTACCGTCGA	AGACAGGTATGACCGGCATA	20	20	55.0	50.0	58.1	57.9	20.3	0.0	0.167506	3789	4448	1228-1228	0.200000	46.7
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	CGAGTACTCTACCGTCGAGT	GCTATTCGGAGGTTCGAGTC	20	20	55.0	55.0	58.1	57.9	20.3	0.0	0.202645	3789	4448	721-721	0.200000	46.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCGGGGCTTTGCAATATTTG	TATAGTTATAGCCCGGCCCC	20	20	50.0	55.0	58.1	58.1	0.0	5.6	0.191310	22477	23277	1286-1490	0.800000	79.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CACGAGCCCATATCCTTTCC	TATAGTTATAGCCCGGCCCC	20	20	55.0	55.0	58.1	58.1	0.0	5.6	0.245700	22477	23277	1408-1612	0.800000	79.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AAGACTAGAGCCCCGCTTAT	TATAGTTATAGCCCGGCCCC	20	20	50.0	55.0	57.9	58.1	0.0	5.6	0.219536	22477	23277	1616-1820	0.800000	78.9
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATGCGAACCCATAGCTGATG	TATAGTTATAGCCCGGCCCC	20	20	50.0	55.0	58.1	58.1	0.0	5.6	0.241721	22477	23277	1464-1668	0.800000	78.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AAAGACTAGAGCCCCGCTTA	TATAGTTATAGCCCGGCCCC	20	20	50.0	55.0	58.1	58.1	0.0	5.6	0.267954	22477	23277	1617-1821	0.800000	78.5
petN-psbM_p1	petN-psbM	1	GTATCATTTTGGCGGCATGG	TCCACCCCGAACTTTTGAAA	20	20	50.0	45.0	57.9	57.6	14.3	0.0	0.577396	23860	24647	1994-2066	0.400000	59.0
petN-psbM_p2	petN-psbM	2	AGGGGGAGGGTTCAAGATAC	TCCACCCCGAACTTTTGAAA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.544101	23860	24647	2082-2082	0.200000	43.6
petN-psbM_p3	petN-psbM	3	AGGGGGAGGGTTCAAGATAC	TTCCACCCCGAACTTTTGAA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.544101	23860	24647	2083-2083	0.200000	43.6
petN-psbM_p4	petN-psbM	4	GGGGCCGGGCTATAACTATA	TCCACCCCGAACTTTTGAAA	20	20	55.0	45.0	58.1	57.6	28.8	0.0	0.568736	23860	24647	1764-1764	0.200000	43.5
petN-psbM_p5	petN-psbM	5	GGGGCCGGGCTATAACTATA	TTCCACCCCGAACTTTTGAA	20	20	55.0	45.0	58.1	57.6	28.8	0.0	0.568736	23860	24647	1765-1765	0.200000	43.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	CGGATTTGAACCAGCGTAGA	TCAGGGCCCCATAGTAACAA	20	20	50.0	50.0	58.0	58.0	0.0	21.4	0.030554	26803	27370	1543-1975	1.000000	89.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	CGGATTTGAACCAGCGTAGA	TTCAGGGCCCCATAGTAACA	20	20	50.0	50.0	58.0	58.0	0.0	21.4	0.030554	26803	27370	1544-1976	1.000000	89.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	CGGATTTGAACCAGCGTAGA	AGCTTGGGTGGGGTTAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014264	26803	27370	1976-2408	1.000000	89.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	CGGATTTGAACCAGCGTAGA	AGTTTCTTCAGCTTGGGTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.017700	26803	27370	1985-2417	1.000000	89.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	CGACCCAGAAGGAATCCTTG	AGCTTGGGTGGGGTTAAAAG	20	20	55.0	50.0	58.0	58.0	20.7	0.0	0.039520	26803	27370	1908-2262	0.600000	68.6
psaA-ycf3_p1	psaA-ycf3	1	CTGGCCCATTCTTCGAAAGA	TGGTTGAAGATCACGAGACG	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.461558	36044	36653	717-798	1.000000	86.4
psaA-ycf3_p2	psaA-ycf3	2	TCTGGCCCATTCTTCGAAAG	TGGTTGAAGATCACGAGACG	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.461558	36044	36653	718-799	1.000000	86.3
psaA-ycf3_p3	psaA-ycf3	3	CTGGCCCATTCTTCGAAAGA	GGTTGAAGATCACGAGACGT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.463157	36044	36653	716-797	1.000000	86.3
psaA-ycf3_p4	psaA-ycf3	4	TCTGGCCCATTCTTCGAAAG	GGTTGAAGATCACGAGACGT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.463157	36044	36653	717-798	1.000000	86.3
psaA-ycf3_p5	psaA-ycf3	5	GTCTGGCCCATTCTTCGAAA	TGGTTGAAGATCACGAGACG	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.526481	36044	36653	719-800	1.000000	85.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CTTCAACAAGCGCGGATTTT	GGAGCCCGCCAATTAGTTAA	20	20	45.0	50.0	57.9	58.0	8.8	0.0	0.097479	38654	39308	1406-1483	1.000000	89.3
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TGAGCGGGGCTAATGTTTTT	GGAGCCCGCCAATTAGTTAA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.129130	38654	39308	1927-1985	1.000000	88.5
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CTTCAACAAGCGCGGATTTT	TTTTCATTTCTTGTCCGCGC	20	20	45.0	45.0	57.9	58.0	8.8	0.0	0.095365	38654	39308	1068-1121	0.800000	79.7
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TGAGCGGGGCTAATGTTTTT	TTTTCATTTCTTGTCCGCGC	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.127016	38654	39308	1563-1622	0.800000	78.9
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	ACAGAAGGGGTTGTGCAATT	TTTTCATTTCTTGTCCGCGC	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.131498	38654	39308	1145-1145	0.200000	46.9
petA-psbJ_p1	petA-psbJ	1	TATGTAGGCGGGAATAGGGG	ACACAATCAAACCCGAACGA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.096719	52257	52778	1308-1362	1.000000	87.4
petA-psbJ_p2	petA-psbJ	2	TATGTAGGCGGGAATAGGGG	ACGACACAATCAAACCCGAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.096719	52257	52778	1311-1365	1.000000	87.4
petA-psbJ_p3	petA-psbJ	3	TATGTAGGCGGGAATAGGGG	ACGGACTAGCTGTACCTACC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.102904	52257	52778	1401-1455	1.000000	87.0
petA-psbJ_p4	petA-psbJ	4	TATGTAGGCGGGAATAGGGG	GGACTAGCTGTACCTACCGT	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.102904	52257	52778	1399-1453	1.000000	87.0
petA-psbJ_p5	petA-psbJ	5	TATGTAGGCGGGAATAGGGG	GAGCCGACAAGGAATTCCAT	20	20	55.0	50.0	58.1	58.0	0.0	26.2	0.097138	52257	52778	1546-1595	1.000000	86.5
psbE-petL_p1	psbE-petL	1	TCGTTCGGGTTTGATTGTGT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.080531	53574	54500	1853-1987	1.000000	89.7
psbE-petL_p2	psbE-petL	2	ACGGTAGGTACAGCTAGTCC	AGAACGTGGGTCTCCAAAAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.086716	53574	54500	1762-1896	1.000000	89.7
psbE-petL_p3	psbE-petL	3	GGTAGGTACAGCTAGTCCGT	AGAACGTGGGTCTCCAAAAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.086716	53574	54500	1760-1894	1.000000	89.7
psbE-petL_p4	psbE-petL	4	ATGGAATTCCTTGTCGGCTC	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.0	58.0	24.7	0.0	0.080951	53574	54500	1620-1704	0.800000	81.0
psbE-petL_p5	psbE-petL	5	TTCGGGTTTGATTGTGTCGT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.080531	53574	54500	1850-1984	0.800000	80.4
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	AGGTGACACTCTACCACTGA	20	20	45.0	50.0	57.7	57.3	16.5	5.4	0.935233	84579	86155	741-1719	1.000000	84.5
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GGTGACACTCTACCACTGAG	20	20	45.0	55.0	57.7	57.0	16.5	5.4	1.277561	84579	86155	740-1718	1.000000	81.8
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCAC	20	21	45.0	52.4	57.7	58.0	16.5	5.4	1.285665	84579	86155	745-1723	1.000000	81.7
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	CAAGGTGACACTCTACCACTG	20	21	45.0	52.4	57.7	58.0	16.5	5.4	1.289708	84579	86155	743-1721	1.000000	81.7
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	AAGGTGACACTCTACCACTGA	20	21	45.0	47.6	57.7	58.0	16.5	5.4	1.298873	84579	86155	742-1720	1.000000	81.6
trnI-GAU-trnA-UGC_p1	trnI-GAU-trnA-UGC	1	CCCGTCCTCAGTTCAGATTG	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	58.0	58.0	0.0	3.6	0.032641	88260	88525	1099-1722	1.000000	91.7
trnI-GAU-trnA-UGC_p2	trnI-GAU-trnA-UGC	2	CTAGTAATCGCCGATCAGCC	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	58.0	58.0	0.0	3.6	0.032932	88260	88525	1045-1668	1.000000	91.7
trnI-GAU-trnA-UGC_p3	trnI-GAU-trnA-UGC	3	CCCGTCCTCAGTTCAGATTG	ATGTTTCAGTTCGCCAGGTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.034479	88260	88525	1166-1789	1.000000	91.7
trnI-GAU-trnA-UGC_p4	trnI-GAU-trnA-UGC	4	CTAGTAATCGCCGATCAGCC	ATGTTTCAGTTCGCCAGGTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.034771	88260	88525	1112-1735	1.000000	91.7
trnI-GAU-trnA-UGC_p5	trnI-GAU-trnA-UGC	5	AACTCCAAAAACCCGTCCTC	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	58.0	58.0	0.0	3.6	0.039849	88260	88525	1110-1733	1.000000	91.7
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	GCGGAAAAATAGCTCGATGC	GCTGCATAGCCCAATAAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037293	92404	92879	1516-2039	0.600000	71.3
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	ATCCCTTCTCCCCCATTTCA	GCTGCATAGCCCAATAAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013407	92404	92879	1396-1928	0.600000	71.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	ATCCCTTCTCCCCCATTTCA	ATAGCGGCATTGTTCCTCAG	20	20	50.0	50.0	58.0	58.0	0.0	1.4	0.041248	92404	92879	780-859	0.400000	64.6
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CCCTGTTCTTACATGACCCG	GCTGCATAGCCCAATAAGGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037293	92404	92879	1832-1832	0.200000	47.7
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	ATCAATTCGATCAGGCACCC	GCTGCATAGCCCAATAAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040246	92404	92879	1812-1812	0.200000	47.7
ccsA-psaC_p1	ccsA-psaC	1	GGGAGCTGTATGGGCTAATG	CATGTATCGGGTGCACTCAA	20	20	55.0	50.0	57.8	58.0	0.0	18.7	0.213892	94095	94640	831-1181	1.000000	90.3
ccsA-psaC_p2	ccsA-psaC	2	GGGAGCTGTATGGGCTAATG	TGTATCGGGTGCACTCAATG	20	20	55.0	50.0	57.8	58.0	0.0	18.7	0.213892	94095	94640	829-1179	1.000000	90.3
ccsA-psaC_p3	ccsA-psaC	3	GGGAGCTGTATGGGCTAATG	GTATCGGGTGCACTCAATGT	20	20	55.0	50.0	57.8	58.0	0.0	18.7	0.215303	94095	94640	828-1178	1.000000	90.3
ccsA-psaC_p4	ccsA-psaC	4	CGCTCTTCAATCCCAATGGT	CATGTATCGGGTGCACTCAA	20	20	50.0	50.0	58.2	58.0	0.0	18.7	0.267585	94095	94640	1164-1514	1.000000	89.9
ccsA-psaC_p5	ccsA-psaC	5	CGCTCTTCAATCCCAATGGT	TGTATCGGGTGCACTCAATG	20	20	50.0	50.0	58.2	58.0	0.0	18.7	0.267585	94095	94640	1162-1512	1.000000	89.9
psaC-ycf1_p1	psaC-ycf1	1	CACATTGAGTGCACCCGATA	TGAACCGATTCGTCTGTCTG	20	20	50.0	50.0	58.0	57.7	14.6	5.2	0.370462	94887	95479	1147-1335	1.000000	89.0
psaC-ycf1_p2	psaC-ycf1	2	CATTGAGTGCACCCGATACA	TGAACCGATTCGTCTGTCTG	20	20	50.0	50.0	58.0	57.7	14.6	5.2	0.370462	94887	95479	1145-1333	1.000000	89.0
psaC-ycf1_p3	psaC-ycf1	3	TTGAGTGCACCCGATACATG	TGAACCGATTCGTCTGTCTG	20	20	50.0	50.0	58.0	57.7	14.6	5.2	0.370462	94887	95479	1143-1331	1.000000	89.0
psaC-ycf1_p4	psaC-ycf1	4	ACATTGAGTGCACCCGATAC	TGAACCGATTCGTCTGTCTG	20	20	50.0	50.0	58.0	57.7	14.6	5.2	0.371873	94887	95479	1146-1334	1.000000	89.0
psaC-ycf1_p5	psaC-ycf1	5	TTGAGTGCACCCGATACATG	GAACCGATTCGTCTGTCTGT	20	20	50.0	50.0	58.0	57.7	14.6	5.2	0.372062	94887	95479	1142-1330	1.000000	89.0
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	GCTGTTAACTGACTGGTCGT	ATCCCTTCTCCCCCATTTCA	20	20	50.0	50.0	57.8	58.0	15.9	0.0	0.150687	100682	101157	738-837	1.000000	90.8
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CGCTGATACCTTCTTCTCGG	ATCCCTTCTCCCCCATTTCA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.127203	100682	101157	1235-1360	0.800000	82.9
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CGCTGATACCTTCTTCTCGG	GCGGAAAAATAGCTCGATGC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.151089	100682	101157	1355-1479	0.800000	82.6
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CGCTGATACCTTCTTCTCGG	CCCTGTTCTTACATGACCCG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.151089	100682	101157	1671-1809	0.600000	71.7
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CGCTGATACCTTCTTCTCGG	ATCAATTCGATCAGGCACCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.154041	100682	101157	1651-1651	0.200000	46.8
trnA-UGC-trnI-GAU_p1	trnA-UGC-trnI-GAU	1	ATTCGGGAATCTCCGGATCT	CCCGTCCTCAGTTCAGATTG	20	20	50.0	55.0	58.0	58.0	3.6	0.0	0.032641	105036	105301	1099-1722	1.000000	91.7
trnA-UGC-trnI-GAU_p2	trnA-UGC-trnI-GAU	2	ATTCGGGAATCTCCGGATCT	CTAGTAATCGCCGATCAGCC	20	20	50.0	55.0	58.0	58.0	3.6	0.0	0.032932	105036	105301	1045-1668	1.000000	91.7
trnA-UGC-trnI-GAU_p3	trnA-UGC-trnI-GAU	3	ATGTTTCAGTTCGCCAGGTT	CCCGTCCTCAGTTCAGATTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.034479	105036	105301	1166-1789	1.000000	91.7
trnA-UGC-trnI-GAU_p4	trnA-UGC-trnI-GAU	4	ATGTTTCAGTTCGCCAGGTT	CTAGTAATCGCCGATCAGCC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.034771	105036	105301	1112-1735	1.000000	91.7
trnA-UGC-trnI-GAU_p5	trnA-UGC-trnI-GAU	5	ATTCGGGAATCTCCGGATCT	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	3.6	0.0	0.039849	105036	105301	1110-1733	1.000000	91.7
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	AGGTGACACTCTACCACTGA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.3	57.7	5.4	16.5	0.935233	107406	108982	741-1719	1.000000	84.5
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GGTGACACTCTACCACTGAG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.0	57.7	5.4	16.5	1.277561	107406	108982	740-1718	1.000000	81.8
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	GTCAAGGTGACACTCTACCAC	TGGCAACTTTACGTAAGGCA	21	20	52.4	45.0	58.0	57.7	5.4	16.5	1.285665	107406	108982	745-1723	1.000000	81.7
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	CAAGGTGACACTCTACCACTG	TGGCAACTTTACGTAAGGCA	21	20	52.4	45.0	58.0	57.7	5.4	16.5	1.289708	107406	108982	743-1721	1.000000	81.7
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	AAGGTGACACTCTACCACTGA	TGGCAACTTTACGTAAGGCA	21	20	47.6	45.0	58.0	57.7	5.4	16.5	1.298873	107406	108982	742-1720	1.000000	81.6