Hotspot

ycf1

SSC · 128225-133774 · 5550 bp

Back to Psidium

Pi 0.00651

MarkerSeek score 53.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5550
variable_sites: 36
pi: 0.00651
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCGCCATTTGTGTGGTACT	AGATCTAAGGTGACACCCCC	45.0	55.0	57.7	58.1	1198 bp	1.0	77.9
ycf1_p2	TTCGCCATTTGTGTGGTACT	GCCTTAAACCTTGGCACAGA	45.0	50.0	57.7	58.4	1215 bp	1.0	75.7
ycf1_p3	TCATCGGAATGCCTTTTGGT	AGATCTAAGGTGACACCCCC	45.0	55.0	57.8	58.1	1259 bp	0.5	57.3
ycf1_p4	TCATCGGAATGCCTTTTGGT	GCCTTAAACCTTGGCACAGA	45.0	50.0	57.8	58.4	1276 bp	0.5	55.2
ycf1_p5	TCATCGGAATGCCTTTTGGT	ACCAGCACAATCAGGAATCA	45.0	45.0	57.8	57.1	770 bp	0.5	51.2