Hotspot

rpoC2

LSC · 17701-21834 · 4134 bp

Back to Psidium

Pi 0.00508

MarkerSeek score 52.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4134
variable_sites: 21
pi: 0.00508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTCTGTTTTAAATTTTCAAGTTGCA	TCAAGGAACTATTCGCACGT	28.0	45.0	56.0	57.3	282 bp	1.0	40.9
rpoC2_p2	AGTCTGTTTTAAATTTTCAAGTTGCA	TCAAGGAACTATTCGCACGT	26.9	45.0	57.2	57.3	283 bp	1.0	40.8
rpoC2_p3	AGTCTGTTTTAAATTTTCAAGTTGCA	TCAAGGAACTATTCGCACGTT	26.9	42.9	57.2	58.0	283 bp	1.0	40.8
rpoC2_p4	AAGTCTGTTTTAAATTTTCAAGTTGCA	TCAAGGAACTATTCGCACGT	25.9	45.0	57.8	57.3	284 bp	1.0	40.8
rpoC2_p5	AGTCTGTTTTAAATTTTCAAGTTGCAA	TCAAGGAACTATTCGCACGT	25.9	45.0	57.8	57.3	283 bp	1.0	40.8