Hotspot

ndhC-trnV-UAC

LSC · 54028-54481 · 454 bp

Back to Psidium

Pi 0.0176

MarkerSeek score 54.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 8
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATCGGGGCTAAAATTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	589-623 bp	1.0	77.0
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	597-631 bp	1.0	76.7
ndhC-trnV-UAC_p3	CGGGGCTAAAATTCCGGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	586-620 bp	1.0	75.2
ndhC-trnV-UAC_p4	AATCGGGGCTAAAATTCCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.3	57.5	588-622 bp	1.0	74.2
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	596-630 bp	1.0	74.0