Hotspot

psbM-trnD-GUC

LSC · 31334-32347 · 1014 bp

Back to Psidium

Pi 0.00701

MarkerSeek score 64.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 7
pi: 0.00701
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	1113-1124 bp	1.0	54.6
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	43.5	50.0	58.0	58.6	1116-1127 bp	1.0	53.1
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TCAATTGGTTAGAGCACCGC	43.5	50.0	57.9	58.6	1120-1131 bp	1.0	52.5
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TCAATTGGTTAGAGCACCGC	40.9	50.0	56.8	58.6	1120-1131 bp	1.0	51.8
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TCAATTGGTTAGAGCACCGC	43.5	50.0	58.2	58.6	1119-1130 bp	1.0	51.8