Hotspot

petN-psbM

LSC · 30270-31228 · 959 bp

Back to Psidium

Pi 0.0177

MarkerSeek score 68.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 959
variable_sites: 17
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	1114-1117 bp	1.0	65.4
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.8	57.0	1120-1123 bp	1.0	63.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	1060-1063 bp	1.0	62.7
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	1114-1117 bp	1.0	61.0
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTC	50.0	45.0	57.8	55.5	1120-1123 bp	1.0	59.6