Hotspot

trnC-GCA-petN

LSC · 29290-30179 · 890 bp

Back to Psidium

Pi 0.00899

MarkerSeek score 63.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 8
pi: 0.00899
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	953-954 bp	1.0	80.0
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	947-948 bp	1.0	78.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	971-972 bp	1.0	77.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1007-1008 bp	1.0	77.2
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	965-966 bp	1.0	76.3