Hotspot

rps18

LSC · 71735-72304 · 570 bp

Back to Pseudoxandra

Pi 0.0175

MarkerSeek score 60.30

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 570
variable_sites: 10
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CCTATTTCGGTCGAATGCGA	ACGCCGCAACAATTAAATCTC	50.0	42.9	58.2	57.9	734-854 bp	1.0	70.7
rps18_p2	TCCTATTTCGGTCGAATGCG	ACGCCGCAACAATTAAATCTC	50.0	42.9	58.2	57.9	735-855 bp	1.0	70.7
rps18_p3	CCTATTTCGGTCGAATGCGA	ACGCCGCAACAATTAAATCT	50.0	40.0	58.2	56.7	734-854 bp	1.0	69.4
rps18_p4	TCCTATTTCGGTCGAATGCG	ACGCCGCAACAATTAAATCT	50.0	40.0	58.2	56.7	735-855 bp	1.0	69.4
rps18_p5	CCTATTTCGGTCGAATGCGA	CGCCGCAACAATTAAATCTCT	50.0	42.9	58.2	57.6	733-853 bp	1.0	68.6