Hotspot

rpl16

LSC · 85558-86976 · 1419 bp

Back to Pseudoxandra

Pi 0.0149

MarkerSeek score 64.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1419
variable_sites: 21
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAATATCCGGTTCCACACCC	ACCTTCAATGAAAAGTGAGCA	50.0	38.1	57.6	56.0	1504-1516 bp	1.0	54.2
rpl16_p2	TGCCAAGGTCGAAGGAAAAA	ACCTTCAATGAAAAGTGAGCA	45.0	38.1	57.9	56.0	1605 bp	0.5	35.4
rpl16_p3	AATGCCAAGGTCGAAGGAAA	ACCTTCAATGAAAAGTGAGCA	45.0	38.1	57.7	56.0	1607 bp	0.5	33.6
rpl16_p4	ATGCCAAGGTCGAAGGAAAA	ACCTTCAATGAAAAGTGAGCA	45.0	38.1	57.7	56.0	1606 bp	0.5	33.6
rpl16_p5	TGCCAAGGTCGAAGGAAAAA	ACCTTCAATGAAAAGTGAGCAA	45.0	36.4	57.9	56.7	1605 bp	0.5	33.3