Hotspot

trnL-CAA-ycf2

IRa · 149198-150240 · 1043 bp

Back to Pseudoxandra

Pi 0.0182

MarkerSeek score 69.20

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 1043
variable_sites: 19
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	AGGCATTGTGGAGAAAGAGA	50.0	45.0	58.0	56.4	1204 bp	1.0	68.7
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	AGGCATTGTGGAGAAAGAGA	50.0	45.0	58.0	56.4	1206 bp	1.0	68.7
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	AGGAGAAGGATTCCCCATTC	50.0	50.0	58.0	56.3	1137 bp	1.0	67.4
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	AGGAGAAGGATTCCCCATTC	50.0	50.0	58.0	56.3	1139 bp	1.0	67.4
trnL-CAA-ycf2_p5	TGGTGAAATGGTAGACACGC	AGGCATTGTGGAGAAAGAGA	50.0	45.0	58.2	56.4	1209 bp	1.0	67.2