Hotspot

rpl32-trnL-UAG

SSC · 117880-119071 · 1192 bp

Back to Pseudoxandra

Pi 0.0369

MarkerSeek score 81.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1192
variable_sites: 43
pi: 0.0369
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGATATTGGGCAGCGGTAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1311 bp	1.0	84.1
rpl32-trnL-UAG_p2	GGATATTGGGCAGCGGTAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1312 bp	1.0	84.1
rpl32-trnL-UAG_p3	GGATATTGGGCAGCGGTAAA	TTTTAGAGATGCCGCCACTC	50.0	50.0	58.0	58.0	1371 bp	1.0	83.8
rpl32-trnL-UAG_p4	GGATATTGGGCAGCGGTAAA	CTTTTAGAGATGCCGCCACT	50.0	50.0	58.0	58.0	1372 bp	1.0	83.7
rpl32-trnL-UAG_p5	GGATATTGGGCAGCGGTAAA	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	1351 bp	1.0	83.5