Hotspot

petA-psbJ

LSC · 66450-67610 · 1161 bp

Back to Pseudoxandra

Pi 0.0201

MarkerSeek score 70.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1161
variable_sites: 22
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GATCCATTACGCGTCCAAGT	CATATTCCGGATTGGGCTCA	50.0	50.0	58.1	57.4	1247-1310 bp	1.0	80.3
petA-psbJ_p2	CGCGTCCAAGTTTTGTTGTT	CATATTCCGGATTGGGCTCA	45.0	50.0	58.1	57.4	1238-1301 bp	1.0	80.1
petA-psbJ_p3	TCCATTACGCGTCCAAGTTT	CATATTCCGGATTGGGCTCA	45.0	50.0	57.8	57.4	1245-1308 bp	1.0	79.5
petA-psbJ_p4	GATCCATTACGCGTCCAAGT	TATTCCGGATTGGGCTCATC	50.0	50.0	58.1	57.1	1245-1308 bp	1.0	78.0
petA-psbJ_p5	GATCCATTACGCGTCCAAGT	TCATATTCCGGATTGGGCTC	50.0	50.0	58.1	57.1	1248-1311 bp	1.0	78.0