Hotspot

rbcL-atpB

LSC · 61600-62396 · 797 bp

Back to Pseudoxandra

Pi 0.0152

MarkerSeek score 68.10

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 12
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	CACCAGCTTTGAATCCAACAC	CATCCAGTACCGGACCAATG	47.6	55.0	58.0	58.0	938-943 bp	1.0	72.9
rbcL-atpB_p2	CACCAGCTTTGAATCCAACAC	CTTCAAGTGTGGAAACCCCA	47.6	50.0	58.0	57.9	890-895 bp	1.0	72.9
rbcL-atpB_p3	ACCAGCTTTGAATCCAACACT	CATCCAGTACCGGACCAATG	42.9	55.0	58.1	58.0	937-942 bp	1.0	72.7
rbcL-atpB_p4	ACCAGCTTTGAATCCAACACT	CTTCAAGTGTGGAAACCCCA	42.9	50.0	58.1	57.9	889-894 bp	1.0	72.6
rbcL-atpB_p5	CACCAGCTTTGAATCCAACAC	TCCAGTACCGGACCAATGAT	47.6	50.0	58.0	58.1	936-941 bp	1.0	72.3