Hotspot

petN-psbM

LSC · 30146-31267 · 1122 bp

Back to Pseudoxandra

Pi 0.0187

MarkerSeek score 69.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1122
variable_sites: 21
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	58.2	58.2	1284-1308 bp	1.0	70.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTC	50.0	45.0	58.2	56.7	1284-1308 bp	1.0	70.1
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	57.6	58.2	1223-1247 bp	1.0	68.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCGTTGTTCATTC	50.0	45.0	57.6	56.7	1223-1247 bp	1.0	68.6
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGTTGTTCATTC	50.0	42.9	58.2	57.5	1285-1309 bp	1.0	67.9