Hotspot

rps16-trnQ-UUG

LSC · 5993-7701 · 1709 bp

Back to Pseudoxandra

Pi 0.0153
MarkerSeek score 63.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1709
variable_sites
26
pi
0.0153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1777-1782 bp 1.0 79.9
rps16-trnQ-UUG_p2 AAATGGAAATGGTGGGTGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.3 58.1 1823-1828 bp 1.0 78.8
rps16-trnQ-UUG_p3 AATGGAAATGGTGGGTGCAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.3 58.1 1822-1827 bp 1.0 78.8
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1781-1786 bp 1.0 78.8
rps16-trnQ-UUG_p5 TCCACAGCAGATCTTGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1799-1804 bp 1.0 78.2