Hotspot

trnH-GUG-psbA

LSC · 87-383 · 297 bp

Back to Pseudoxandra

Pi 0.0539

MarkerSeek score 81.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 297
variable_sites: 16
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TCACAATCCACTGCCTTGAG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.8	57.9	381 bp	1.0	81.3
trnH-GUG-psbA_p2	TCACAATCCACTGCCTTGAG	TGCTGTCGAAGTTCCATCTG	50.0	50.0	57.8	57.9	367 bp	1.0	81.3
trnH-GUG-psbA_p3	TCACAATCCACTGCCTTGAG	GCTGTCGAAGTTCCATCTGT	50.0	50.0	57.8	57.9	366 bp	1.0	81.3
trnH-GUG-psbA_p4	TCACAATCCACTGCCTTGAG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.8	57.9	384 bp	1.0	81.2
trnH-GUG-psbA_p5	TCACAATCCACTGCCTTGAG	TAGACCTAGCTGCTGTCGAA	50.0	50.0	57.8	57.9	377 bp	1.0	80.9