Hotspot

tRNA-Ser

Genome · 31532-31616 · 85 bp

Back to Pseudotsuga

Pi 0.0583

MarkerSeek score 62.10

Primer available yes

Label tRNA-Ser

Feature Metadata

label_name: tRNA-Ser
feature_type: tRNA
region: Genome
length_bp: 85
variable_sites: 7
pi: 0.0583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser_p1	TTGAAATCCGTTGGCAAGGA	CGTGAGCGCTAAGTCGATTA	45.0	50.0	58.0	57.9	443-484 bp	1.0	87.1
tRNA-Ser_p2	TTGAAATCCGTTGGCAAGGA	TTGACAGGGCGGTACTCTAA	45.0	50.0	58.0	58.1	595-621 bp	1.0	86.4
tRNA-Ser_p3	TTGAAATCCGTTGGCAAGGA	AGGGCGGTACTCTAACCAAT	45.0	50.0	58.0	58.1	590-616 bp	1.0	86.0
tRNA-Ser_p4	TTGAAATCCGTTGGCAAGGA	CCACTCATGACCCTGGAATG	45.0	55.0	58.0	58.0	1023-1049 bp	1.0	85.7
tRNA-Ser_p5	CACGAGGACGGACTTGAAAT	CCACTCATGACCCTGGAATG	50.0	55.0	57.9	58.0	941-1381 bp	1.0	82.5