Hotspot

tRNA-His-tRNA-Ile

Genome · 121120-121412 · 293 bp

Back to Pseudotsuga

Pi 0.0614

MarkerSeek score 77.60

Primer available yes

Label tRNA-His-tRNA-Ile

Feature Metadata

label_name: tRNA-His-tRNA-Ile
feature_type: igs
region: Genome
length_bp: 293
variable_sites: 27
pi: 0.0614
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-His-tRNA-Ile_p1	TCACAATCCACTGCCTTGTC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	363-373 bp	1.0	84.9
tRNA-His-tRNA-Ile_p2	TTCACAATCCACTGCCTTGT	GTTGGGCGCTTTAACCATTC	45.0	50.0	57.6	58.0	364-374 bp	1.0	82.8
tRNA-His-tRNA-Ile_p3	TCACAATCCACTGCCTTGTC	AGTTGGGCGCTTTAACCATT	50.0	45.0	58.1	58.4	364-374 bp	1.0	82.0
tRNA-His-tRNA-Ile_p4	TCACAATCCACTGCCTTGTC	TTGGGCGCTTTAACCATTCA	50.0	45.0	58.1	58.4	362-372 bp	1.0	82.0
tRNA-His-tRNA-Ile_p5	CTTGTCCACTTGGCTACGTC	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.6	58.0	349-359 bp	1.0	81.4