Hotspot

rps7-tRNA-Leu

Genome · 112368-113763 · 1396 bp

Back to Pseudotsuga

Pi 0.0149

MarkerSeek score 60.00

Primer available yes

Label rps7-tRNA-Leu

Feature Metadata

label_name: rps7-tRNA-Leu
feature_type: igs
region: Genome
length_bp: 1396
variable_sites: 31
pi: 0.0149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-tRNA-Leu_p1	CAGAGGGAATGGCAATGCTA	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.6	58.0	1513-1928 bp	1.0	78.9
rps7-tRNA-Leu_p2	TTAATGGATGCTGCCAGAGG	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.6	58.0	1527-1942 bp	1.0	78.9
rps7-tRNA-Leu_p3	CAGAGGGAATGGCAATGCTA	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.6	58.0	1511-1926 bp	1.0	78.9
rps7-tRNA-Leu_p4	TTAATGGATGCTGCCAGAGG	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.6	58.0	1525-1940 bp	1.0	78.9
rps7-tRNA-Leu_p5	ACGCAAAAAGGAAGAGACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.4	58.0	1492-1907 bp	1.0	78.6