Hotspot

tRNA-Ile-tRNA-Phe

Genome · 66970-68910 · 1941 bp

Back to Pseudotsuga

Pi 0.00996
MarkerSeek score 57.10
Primer available yes
Label tRNA-Ile-tRNA-Phe

Feature Metadata

label_name
tRNA-Ile-tRNA-Phe
feature_type
igs
region
Genome
length_bp
1941
variable_sites
29
pi
0.00996
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Ile-tRNA-Phe_p1 GTTGGGCGCTTTAACCATTC CCTCGTGTCACCAGTTCAAA 50.0 50.0 58.0 58.1 2007-2021 bp 1.0 80.7
tRNA-Ile-tRNA-Phe_p2 GTTGGGCGCTTTAACCATTC TACCAGGATGATCCACAGGG 50.0 55.0 58.0 58.2 2070-2084 bp 1.0 79.5
tRNA-Ile-tRNA-Phe_p3 AGTTGGGCGCTTTAACCATT CCTCGTGTCACCAGTTCAAA 45.0 50.0 58.4 58.1 2008-2022 bp 1.0 77.8
tRNA-Ile-tRNA-Phe_p4 TTGGGCGCTTTAACCATTCA CCTCGTGTCACCAGTTCAAA 45.0 50.0 58.4 58.1 2006-2020 bp 1.0 77.7
tRNA-Ile-tRNA-Phe_p5 GTTGGGCGCTTTAACCATTC AATCCTCGTGTCACCAGTTC 50.0 50.0 58.0 57.5 2010-2024 bp 1.0 77.4