Hotspot

infA-rps8

Genome · 60776-60917 · 142 bp

Back to Pseudotsuga

Pi 0.0146

MarkerSeek score 64.90

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: Genome
length_bp: 142
variable_sites: 3
pi: 0.0146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ACTCGAAACATACCGTTGGG	AGGTGGAATGGGGATCGTAA	50.0	50.0	57.9	58.1	314-319 bp	1.0	80.4
infA-rps8_p2	TCAGTAACCAAACCTTCCGC	AGGTGGAATGGGGATCGTAA	50.0	50.0	58.1	58.1	287-292 bp	1.0	80.4
infA-rps8_p3	AAACCTTCCGCATGGATCAA	AGGTGGAATGGGGATCGTAA	45.0	50.0	57.8	58.1	278-283 bp	1.0	79.7
infA-rps8_p4	AACCTTCCGCATGGATCAAA	AGGTGGAATGGGGATCGTAA	45.0	50.0	57.8	58.1	277-282 bp	1.0	79.7
infA-rps8_p5	AAACATACCGTTGGGGAGTG	AGGTGGAATGGGGATCGTAA	50.0	50.0	57.8	58.1	309-314 bp	1.0	79.6