Hotspot

matK-tRNA-Lys

Genome · 3371-4154 · 784 bp

Back to Pseudotsuga

Pi 0.0068
MarkerSeek score 63.10
Primer available yes
Label matK-tRNA-Lys

Feature Metadata

label_name
matK-tRNA-Lys
feature_type
igs
region
Genome
length_bp
784
variable_sites
8
pi
0.0068
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-tRNA-Lys_p1 TGTTGCCAAGAGCTATCTTCC TAACCTCGCATTCCACTTCG 47.6 50.0 58.0 58.0 921 bp 1.0 72.6
matK-tRNA-Lys_p2 GCATTGTTGCCAAGAGCTATC TAACCTCGCATTCCACTTCG 47.6 50.0 57.9 58.0 925 bp 1.0 72.0
matK-tRNA-Lys_p3 TGTTGCCAAGAGCTATCTTCC CGCATTCCACTTCGATCGTA 47.6 50.0 58.0 58.2 915 bp 1.0 71.3
matK-tRNA-Lys_p4 GCATTGTTGCCAAGAGCTATC CGCATTCCACTTCGATCGTA 47.6 50.0 57.9 58.2 919 bp 1.0 70.8
matK-tRNA-Lys_p5 GCATTGTTGCCAAGAGCTAT TAACCTCGCATTCCACTTCG 45.0 50.0 56.8 58.0 925 bp 1.0 70.7