primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-tRNA-Lys_p1	matK-tRNA-Lys	1	TGTTGCCAAGAGCTATCTTCC	TAACCTCGCATTCCACTTCG	21	20	47.6	50.0	58.0	58.0	0.0	0.0	1.013332	3371	4154	921-921	1.000000	72.6
matK-tRNA-Lys_p2	matK-tRNA-Lys	2	GCATTGTTGCCAAGAGCTATC	TAACCTCGCATTCCACTTCG	21	20	47.6	50.0	57.9	58.0	0.0	0.0	1.080448	3371	4154	925-925	1.000000	72.0
matK-tRNA-Lys_p3	matK-tRNA-Lys	3	TGTTGCCAAGAGCTATCTTCC	CGCATTCCACTTCGATCGTA	21	20	47.6	50.0	58.0	58.2	0.0	15.9	1.173629	3371	4154	915-915	1.000000	71.3
matK-tRNA-Lys_p4	matK-tRNA-Lys	4	GCATTGTTGCCAAGAGCTATC	CGCATTCCACTTCGATCGTA	21	20	47.6	50.0	57.9	58.2	0.0	15.9	1.240745	3371	4154	919-919	1.000000	70.8
matK-tRNA-Lys_p5	matK-tRNA-Lys	5	GCATTGTTGCCAAGAGCTAT	TAACCTCGCATTCCACTTCG	20	20	45.0	50.0	56.8	58.0	0.0	0.0	1.246481	3371	4154	925-925	1.000000	70.7
ycf12-clpP_p1	ycf12-clpP	1	AGTCGATGGGATCATAGGGG	AGCATCGTTACGTGTTTCGA	20	20	55.0	45.0	58.1	57.9	0.0	4.2	0.110510	8634	9052	1004-1092	1.000000	81.5
ycf12-clpP_p2	ycf12-clpP	2	AGTCGATGGGATCATAGGGG	CTCGTTGAATGACCTCTCCG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.141189	8634	9052	1069-1157	1.000000	81.2
ycf12-clpP_p3	ycf12-clpP	3	AGTCGATGGGATCATAGGGG	CGTTATGAAAATCTGCGGCC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.161146	8634	9052	982-1070	1.000000	81.1
ycf12-clpP_p4	ycf12-clpP	4	AGTCGATGGGATCATAGGGG	GTTATGAAAATCTGCGGCCG	20	20	55.0	50.0	58.1	57.9	0.0	24.0	0.161146	8634	9052	981-1069	1.000000	81.1
ycf12-clpP_p5	ycf12-clpP	5	GGTTGTATCGGGCCCATTAG	AGCATCGTTACGTGTTTCGA	20	20	55.0	45.0	58.1	57.9	24.9	4.2	0.162478	8634	9052	926-926	0.333333	52.0
petL-psbE_p1	petL-psbE	1	GCGTGGAAGGACTGAATGAA	AATGTCAGCAAAGGAGCGTT	20	20	50.0	45.0	58.2	58.4	0.0	0.0	0.582381	13452	14660	1091-1303	1.000000	79.8
petL-psbE_p2	petL-psbE	2	GCGTGGAAGGACTGAATGAA	GTATTCCCAGCCATGCTCAG	20	20	50.0	55.0	58.2	58.4	0.0	0.0	0.589279	13452	14660	1066-1278	1.000000	79.7
petL-psbE_p3	petL-psbE	3	GCGTGGAAGGACTGAATGAA	TAATGTCAGCAAAGGAGCGT	20	20	50.0	45.0	58.2	57.5	0.0	0.0	0.664285	13452	14660	1092-1304	1.000000	79.1
petL-psbE_p4	petL-psbE	4	TAGGCGTGGAAGGACTGAAT	AATGTCAGCAAAGGAGCGTT	20	20	50.0	45.0	58.4	58.4	0.0	0.0	0.822288	13452	14660	1094-1306	1.000000	77.8
petL-psbE_p5	petL-psbE	5	TAGGCGTGGAAGGACTGAAT	GTATTCCCAGCCATGCTCAG	20	20	50.0	55.0	58.4	58.4	0.0	0.0	0.829185	13452	14660	1069-1281	1.000000	77.8
psbJ-petA_p1	psbJ-petA	1	TGGAAGGATCCCTCTTTGGT	AGCATCTGTCATTCTGGCAC	20	20	50.0	50.0	57.9	58.3	8.6	0.0	0.312047	15438	16485	1242-1288	1.000000	78.5
psbJ-petA_p2	psbJ-petA	2	TACCACTGGAAGGATCCCTC	AGCATCTGTCATTCTGGCAC	20	20	55.0	50.0	57.8	58.3	8.6	0.0	0.428538	15438	16485	1248-1294	1.000000	77.6
psbJ-petA_p3	psbJ-petA	3	TGGAAGGATCCCTCTTTGGT	GCATCTGTCATTCTGGCACA	20	20	50.0	50.0	57.9	58.5	8.6	0.0	0.603460	15438	16485	1241-1287	1.000000	76.2
psbJ-petA_p4	psbJ-petA	4	CCACTGGAAGGATCCCTCTT	AGCATCTGTCATTCTGGCAC	20	20	55.0	50.0	58.4	58.3	8.6	0.0	0.662876	15438	16485	1246-1292	1.000000	75.7
psbJ-petA_p5	psbJ-petA	5	TACCACTGGAAGGATCCCTC	GCATCTGTCATTCTGGCACA	20	20	55.0	50.0	57.8	58.5	8.6	0.0	0.719951	15438	16485	1247-1293	1.000000	75.2
ycf4-psaI_p1	ycf4-psaI	1	GAGCCATTTGGATCGACGAT	TTTCCGCTCTCCGAAATCAG	20	20	50.0	50.0	58.1	58.0	10.8	0.0	0.139065	19843	20385	1022-1025	1.000000	80.5
ycf4-psaI_p2	ycf4-psaI	2	AGGGGTATCAGGTTCTCACC	TTTCCGCTCTCCGAAATCAG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.129204	19843	20385	1150-1153	1.000000	80.4
ycf4-psaI_p3	ycf4-psaI	3	ACTCGAAATACCGACCAGGA	TTTCCGCTCTCCGAAATCAG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.166777	19843	20385	1124-1127	1.000000	80.2
ycf4-psaI_p4	ycf4-psaI	4	CGACGAGGGGAAATACCTTC	TTTCCGCTCTCCGAAATCAG	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.230624	19843	20385	1417-1420	1.000000	79.5
ycf4-psaI_p5	ycf4-psaI	5	TTCAGCAGCTTTCTGTTCGA	TTTCCGCTCTCCGAAATCAG	20	20	45.0	50.0	57.8	58.0	11.3	0.0	0.249087	19843	20385	1514-1517	1.000000	79.3
tRNA-Thr-tRNA-Ser_p1	tRNA-Thr-tRNA-Ser	1	GAACCGATGACTTACGCCTT	TAGTGACCGAACGGAGAGAG	20	20	50.0	55.0	58.0	58.3	0.0	20.1	0.270584	29209	30388	1334-1347	1.000000	79.2
tRNA-Thr-tRNA-Ser_p2	tRNA-Thr-tRNA-Ser	2	GAACCGATGACTTACGCCTT	CGGAGAGAGAGGGATTCGAA	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.324433	29209	30388	1323-1336	1.000000	78.8
tRNA-Thr-tRNA-Ser_p3	tRNA-Thr-tRNA-Ser	3	CCATGGCGTTACTCTACCAC	TAGTGACCGAACGGAGAGAG	20	20	55.0	55.0	58.1	58.3	0.0	20.1	0.328681	29209	30388	1313-1326	1.000000	78.8
tRNA-Thr-tRNA-Ser_p4	tRNA-Thr-tRNA-Ser	4	TGGCGTTACTCTACCACTGA	TAGTGACCGAACGGAGAGAG	20	20	50.0	55.0	58.1	58.3	0.0	20.1	0.350836	29209	30388	1310-1323	1.000000	78.6
tRNA-Thr-tRNA-Ser_p5	tRNA-Thr-tRNA-Ser	5	CGTGTAAGACGGACATCCAG	TAGTGACCGAACGGAGAGAG	20	20	55.0	55.0	58.1	58.3	0.0	20.1	0.355238	29209	30388	1399-1412	1.000000	78.5
tRNA-Tyr-tRNA-Ser_p1	tRNA-Tyr-tRNA-Ser	1	CTGGTTCAAATCCAGCTCGA	ATTTGAACCAGCGTAGGCAT	20	20	50.0	45.0	57.9	57.9	0.0	10.5	0.227912	31235	31531	323-577	1.000000	86.4
tRNA-Tyr-tRNA-Ser_p2	tRNA-Tyr-tRNA-Ser	2	CTGGTTCAAATCCAGCTCGA	TGGATTTGAACCAGCGTAGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.207091	31235	31531	396-669	1.000000	86.1
tRNA-Tyr-tRNA-Ser_p3	tRNA-Tyr-tRNA-Ser	3	AGTGGACCAGGACATCTCTC	TTGACAGGGCGGTACTCTAA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.213211	31235	31531	892-1216	1.000000	84.5
tRNA-Tyr-tRNA-Ser_p4	tRNA-Tyr-tRNA-Ser	4	GTGGACCAGGACATCTCTCT	TTGACAGGGCGGTACTCTAA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.213211	31235	31531	891-1215	1.000000	84.5
tRNA-Tyr-tRNA-Ser_p5	tRNA-Tyr-tRNA-Ser	5	CACGTGCGTGTGAAAAGAAA	TTGACAGGGCGGTACTCTAA	20	20	45.0	50.0	57.8	58.1	15.6	0.0	0.239254	31235	31531	1121-1440	1.000000	84.3
tRNA-Ser_p1	tRNA-Ser	1	TTGAAATCCGTTGGCAAGGA	CGTGAGCGCTAAGTCGATTA	20	20	45.0	50.0	58.0	57.9	20.6	11.5	0.112759	31532	31616	443-484	1.000000	87.1
tRNA-Ser_p2	tRNA-Ser	2	TTGAAATCCGTTGGCAAGGA	TTGACAGGGCGGTACTCTAA	20	20	45.0	50.0	58.0	58.1	20.6	0.0	0.084219	31532	31616	595-621	1.000000	86.4
tRNA-Ser_p3	tRNA-Ser	3	TTGAAATCCGTTGGCAAGGA	AGGGCGGTACTCTAACCAAT	20	20	45.0	50.0	58.0	58.1	20.6	0.0	0.136218	31532	31616	590-616	1.000000	86.0
tRNA-Ser_p4	tRNA-Ser	4	TTGAAATCCGTTGGCAAGGA	CCACTCATGACCCTGGAATG	20	20	45.0	55.0	58.0	58.0	20.6	0.0	0.060151	31532	31616	1023-1049	1.000000	85.7
tRNA-Ser_p5	tRNA-Ser	5	CACGAGGACGGACTTGAAAT	CCACTCATGACCCTGGAATG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.128730	31532	31616	941-1381	1.000000	82.5
tRNA-Asp-psbM_p1	tRNA-Asp-psbM	1	TTAGAGTACCGCCCTGTCAA	AGCTCGCTCGAGATTTGATC	20	20	50.0	50.0	58.1	57.9	0.0	18.7	0.205253	31951	32682	835-840	1.000000	79.3
tRNA-Asp-psbM_p2	tRNA-Asp-psbM	2	TTAGAGTACCGCCCTGTCAA	GCTCGCTCGAGATTTGATCT	20	20	50.0	50.0	58.1	57.9	0.0	18.7	0.205253	31951	32682	834-839	1.000000	79.3
tRNA-Asp-psbM_p3	tRNA-Asp-psbM	3	ATTGGTTAGAGTACCGCCCT	AGCTCGCTCGAGATTTGATC	20	20	50.0	50.0	58.1	57.9	0.0	18.7	0.257252	31951	32682	840-845	1.000000	78.9
tRNA-Asp-psbM_p4	tRNA-Asp-psbM	4	ATTGGTTAGAGTACCGCCCT	GCTCGCTCGAGATTTGATCT	20	20	50.0	50.0	58.1	57.9	0.0	18.7	0.257252	31951	32682	839-844	1.000000	78.9
tRNA-Asp-psbM_p5	tRNA-Asp-psbM	5	TAGAGTACCGCCCTGTCAAG	GCTCGCTCGAGATTTGATCT	20	20	55.0	50.0	58.5	57.9	0.0	18.7	0.661621	31951	32682	833-838	1.000000	75.7
infA-rps8_p1	infA-rps8	1	ACTCGAAACATACCGTTGGG	AGGTGGAATGGGGATCGTAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.201152	60776	60917	314-319	1.000000	80.4
infA-rps8_p2	infA-rps8	2	TCAGTAACCAAACCTTCCGC	AGGTGGAATGGGGATCGTAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.225316	60776	60917	287-292	1.000000	80.4
infA-rps8_p3	infA-rps8	3	AAACCTTCCGCATGGATCAA	AGGTGGAATGGGGATCGTAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.326579	60776	60917	278-283	1.000000	79.7
infA-rps8_p4	infA-rps8	4	AACCTTCCGCATGGATCAAA	AGGTGGAATGGGGATCGTAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.326579	60776	60917	277-282	1.000000	79.7
infA-rps8_p5	infA-rps8	5	AAACATACCGTTGGGGAGTG	AGGTGGAATGGGGATCGTAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.306404	60776	60917	309-314	1.000000	79.6
tRNA-Ile-tRNA-Phe_p1	tRNA-Ile-tRNA-Phe	1	GTTGGGCGCTTTAACCATTC	CCTCGTGTCACCAGTTCAAA	20	20	50.0	50.0	58.0	58.1	7.6	0.0	0.061097	66970	68910	2007-2021	1.000000	80.7
tRNA-Ile-tRNA-Phe_p2	tRNA-Ile-tRNA-Phe	2	GTTGGGCGCTTTAACCATTC	TACCAGGATGATCCACAGGG	20	20	50.0	55.0	58.0	58.2	7.6	14.9	0.202705	66970	68910	2070-2084	1.000000	79.5
tRNA-Ile-tRNA-Phe_p3	tRNA-Ile-tRNA-Phe	3	AGTTGGGCGCTTTAACCATT	CCTCGTGTCACCAGTTCAAA	20	20	45.0	50.0	58.4	58.1	7.6	0.0	0.424032	66970	68910	2008-2022	1.000000	77.8
tRNA-Ile-tRNA-Phe_p4	tRNA-Ile-tRNA-Phe	4	TTGGGCGCTTTAACCATTCA	CCTCGTGTCACCAGTTCAAA	20	20	45.0	50.0	58.4	58.1	7.6	0.0	0.429904	66970	68910	2006-2020	1.000000	77.7
tRNA-Ile-tRNA-Phe_p5	tRNA-Ile-tRNA-Phe	5	GTTGGGCGCTTTAACCATTC	AATCCTCGTGTCACCAGTTC	20	20	50.0	50.0	58.0	57.5	7.6	0.0	0.466567	66970	68910	2010-2024	1.000000	77.4
tRNA-Met-tRNA-Gly_p1	tRNA-Met-tRNA-Gly	1	CTTGAGGTCACGGGTTCAAA	ATGCGGGTATGGTTGAATGG	20	20	50.0	50.0	58.0	58.3	4.2	0.0	0.347010	80052	80216	259-271	1.000000	82.4
tRNA-Met-tRNA-Gly_p2	tRNA-Met-tRNA-Gly	2	CTTGAGGTCACGGGTTCAAA	GCGGGTATGGTTGAATGGTA	20	20	50.0	50.0	58.0	57.7	4.2	0.0	0.373522	80052	80216	257-269	1.000000	82.2
tRNA-Met-tRNA-Gly_p3	tRNA-Met-tRNA-Gly	3	TGCGGAGTAGAGTAGTCTGG	ATGCGGGTATGGTTGAATGG	20	20	55.0	50.0	58.0	58.3	10.5	0.0	0.348622	80052	80216	299-311	1.000000	81.7
tRNA-Met-tRNA-Gly_p4	tRNA-Met-tRNA-Gly	4	GCGGAGTAGAGTAGTCTGGT	ATGCGGGTATGGTTGAATGG	20	20	55.0	50.0	58.0	58.3	10.5	0.0	0.350055	80052	80216	298-310	1.000000	81.7
tRNA-Met-tRNA-Gly_p5	tRNA-Met-tRNA-Gly	5	GGTGCGGAGTAGAGTAGTCT	ATGCGGGTATGGTTGAATGG	20	20	55.0	50.0	58.0	58.3	5.2	0.0	0.350055	80052	80216	301-313	1.000000	81.7
chlN-rps15_p1	chlN-rps15	1	ACAAAAATGATCGCTCTGT	ATAATGGGCCATGGTTGCTC	19	20	36.8	50.0	52.8	58.3	0.0	22.0	6.443360	97573	97722	214-241	1.000000	42.8
chlN-rps15_p2	chlN-rps15	2	ACAAAAATGATCGCTCTGT	CATAATGGGCCATGGTTGCT	19	20	36.8	50.0	52.8	58.6	0.0	22.0	6.735458	97573	97722	215-242	1.000000	42.7
chlN-rps15_p3	chlN-rps15	3	ACAAAAATGATCGCTCTGT	ACATAATGGGCCATGGTTGC	19	20	36.8	50.0	52.8	58.9	0.0	22.0	7.029475	97573	97722	216-243	1.000000	42.7
chlN-rps15_p4	chlN-rps15	4	ACAAAAATGATCGCTCTGT	ATCGTATCGGCCGATAAGCT	19	20	36.8	50.0	52.8	58.8	0.0	39.6	6.976919	97573	97722	252-279	1.000000	42.4
chlN-rps15_p5	chlN-rps15	5	ACAAAAATGATCGCTCTGT	TATCGTATCGGCCGATAAGC	19	20	36.8	50.0	52.8	57.2	0.0	39.6	6.990088	97573	97722	253-280	1.000000	42.4
rps15-psaC_p1	rps15-psaC	1	GGGCAAACGTAAGCAACTTC	GTACGCATTGGGTACAACCA	20	20	50.0	50.0	58.0	58.2	13.3	29.0	0.235324	103406	104740	1493-1503	1.000000	78.9
rps15-psaC_p2	rps15-psaC	2	TTTGGGCAAACGTAAGCAAC	GTACGCATTGGGTACAACCA	20	20	45.0	50.0	57.8	58.2	13.3	29.0	0.400394	103406	104740	1496-1506	1.000000	77.6
rps15-psaC_p3	rps15-psaC	3	GGGCAAACGTAAGCAACTTC	ATCTGTGGGACAAGCTCGTA	20	20	50.0	50.0	58.0	58.4	13.3	0.0	0.489184	103406	104740	1510-1520	1.000000	76.9
rps15-psaC_p4	rps15-psaC	4	TTTGGGCAAACGTAAGCAAC	ATCTGTGGGACAAGCTCGTA	20	20	45.0	50.0	57.8	58.4	13.3	0.0	0.654254	103406	104740	1513-1523	1.000000	75.6
rps15-psaC_p5	rps15-psaC	5	AAATTGGGTATTCGTGGGCT	GTACGCATTGGGTACAACCA	20	20	45.0	50.0	57.5	58.2	0.0	29.0	0.714146	103406	104740	1423-1433	1.000000	75.1
rps12_p1	rps12	1	ATCGAACGAGAAGCCGTATG	GTACTTCGCCGTGACATGAG	20	20	50.0	55.0	57.9	58.7	2.9	0.0	0.851950	111047	111847	955-955	1.000000	73.8
rps12_p2	rps12	2	AAGCCGTATGAGGTGCAAAT	GTACTTCGCCGTGACATGAG	20	20	45.0	55.0	57.9	58.7	0.0	0.0	0.859605	111047	111847	945-945	1.000000	73.7
rps12_p3	rps12	3	GAACGAGAAGCCGTATGAGG	GTACTTCGCCGTGACATGAG	20	20	55.0	55.0	58.2	58.7	0.0	0.0	0.889385	111047	111847	952-952	1.000000	73.5
rps12_p4	rps12	4	ATCGAACGAGAAGCCGTATG	GCCGTGACATGAGTGTGAAA	20	20	50.0	50.0	57.9	58.8	2.9	0.0	0.895261	111047	111847	948-948	1.000000	73.4
rps12_p5	rps12	5	AAGCCGTATGAGGTGCAAAT	GCCGTGACATGAGTGTGAAA	20	20	45.0	50.0	57.9	58.8	0.0	0.0	0.902916	111047	111847	938-938	1.000000	73.4
rps7-tRNA-Leu_p1	rps7-tRNA-Leu	1	CAGAGGGAATGGCAATGCTA	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.6	58.0	4.8	5.8	0.361187	112368	113763	1513-1928	1.000000	78.9
rps7-tRNA-Leu_p2	rps7-tRNA-Leu	2	TTAATGGATGCTGCCAGAGG	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.6	58.0	0.0	5.8	0.361187	112368	113763	1527-1942	1.000000	78.9
rps7-tRNA-Leu_p3	rps7-tRNA-Leu	3	CAGAGGGAATGGCAATGCTA	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.6	58.0	4.8	5.8	0.362557	112368	113763	1511-1926	1.000000	78.9
rps7-tRNA-Leu_p4	rps7-tRNA-Leu	4	TTAATGGATGCTGCCAGAGG	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	5.8	0.362557	112368	113763	1525-1940	1.000000	78.9
rps7-tRNA-Leu_p5	rps7-tRNA-Leu	5	ACGCAAAAAGGAAGAGACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.4	58.0	0.0	5.8	0.402078	112368	113763	1492-1907	1.000000	78.6
tRNA-His-tRNA-Ile_p1	tRNA-His-tRNA-Ile	1	TCACAATCCACTGCCTTGTC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.112981	121120	121412	363-373	1.000000	84.9
tRNA-His-tRNA-Ile_p2	tRNA-His-tRNA-Ile	2	TTCACAATCCACTGCCTTGT	GTTGGGCGCTTTAACCATTC	20	20	45.0	50.0	57.6	58.0	0.0	7.6	0.372507	121120	121412	364-374	1.000000	82.8
tRNA-His-tRNA-Ile_p3	tRNA-His-tRNA-Ile	3	TCACAATCCACTGCCTTGTC	AGTTGGGCGCTTTAACCATT	20	20	50.0	45.0	58.1	58.4	0.0	7.6	0.475917	121120	121412	364-374	1.000000	82.0
tRNA-His-tRNA-Ile_p4	tRNA-His-tRNA-Ile	4	TCACAATCCACTGCCTTGTC	TTGGGCGCTTTAACCATTCA	20	20	50.0	45.0	58.1	58.4	0.0	7.6	0.481788	121120	121412	362-372	1.000000	82.0
tRNA-His-tRNA-Ile_p5	tRNA-His-tRNA-Ile	5	CTTGTCCACTTGGCTACGTC	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.6	58.0	0.0	7.6	0.579738	121120	121412	349-359	1.000000	81.4