Hotspot

ndhF

SSC · 106941-109172 · 2232 bp

Back to Pseudostellaria

Pi 0.00815

MarkerSeek score 49.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 45
pi: 0.00815
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGGGAATCAACATTCAATCTGA	ACATACTAAACCACGCCGAT	39.1	45.0	58.1	57.0	2725-2742 bp	0.857143	44.2
ndhF_p2	TGGGGAATCAACATTCAATCTGA	TGTACATACTAAACCACGCCG	39.1	47.6	58.1	58.1	2728-2745 bp	0.857143	43.0
ndhF_p3	TGGGGAATCAACATTCAATCTGA	GTACATACTAAACCACGCCGA	39.1	47.6	58.1	57.9	2727-2744 bp	0.857143	42.8
ndhF_p4	TGGGGAATCAACATTCAATCTG	ACATACTAAACCACGCCGAT	40.9	45.0	56.7	57.0	2725-2742 bp	0.857143	41.9
ndhF_p5	TGGGGAATCAACATTCAATCTG	TGTACATACTAAACCACGCCG	40.9	47.6	56.7	58.1	2728-2745 bp	0.857143	40.7