Hotspot

ndhA

SSC · 116423-118474 · 2052 bp

Back to Pseudostellaria

Pi 0.01

MarkerSeek score 43.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2052
variable_sites: 54
pi: 0.01
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCGTTCTGAGGCGATCAATT	AGTCCAGTGGCAAAAGGAAG	50.0	50.0	58.3	58.0	2728-2737 bp	0.857143	73.7
ndhA_p2	CCGTTCTGAGGCGATCAATT	GGGGCTTATCAGGACCAATG	50.0	55.0	58.3	58.0	2822-2831 bp	0.857143	73.7
ndhA_p3	CCGTTCTGAGGCGATCAATT	GAAGTCCAGTGGCAAAAGGA	50.0	50.0	58.3	58.0	2730-2739 bp	0.857143	73.7
ndhA_p4	CCGTTCTGAGGCGATCAATT	TTAGAACGAGTTGAGGGGGT	50.0	50.0	58.3	58.0	2874-2883 bp	0.857143	73.6
ndhA_p5	CCGTTCTGAGGCGATCAATT	TGGCAAAAGGAAGGGGATTC	50.0	50.0	58.3	58.1	2721-2730 bp	0.857143	73.4