Hotspot

rpl32-trnL-UAG

SSC · 110069-110890 · 822 bp

Back to Pseudostellaria

Pi 0.0225
MarkerSeek score 73.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
822
variable_sites
48
pi
0.0225
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAAAATCCCTTTATACCGGA TCCTAAGAGCAGCGTGTCTA 40.9 50.0 57.5 57.9 905-937 bp 1.0 67.0
rpl32-trnL-UAG_p2 AGCAAAATCCCTTTATACCGGA AGCAGCGTGTCTACCAATTT 40.9 45.0 57.5 57.8 898-930 bp 1.0 66.6
rpl32-trnL-UAG_p3 AGCAAAATCCCTTTATACCGGA CCTAAGAGCAGCGTGTCTAC 40.9 55.0 57.5 57.8 904-936 bp 1.0 66.5
rpl32-trnL-UAG_p4 AGCAAAATCCCTTTATACCGGA CTAAGAGCAGCGTGTCTACC 40.9 55.0 57.5 57.8 903-935 bp 1.0 66.5
rpl32-trnL-UAG_p5 AGCAAAATCCCTTTATACCGGA GCTATCGCACTGCTTCCTAA 40.9 50.0 57.5 57.8 919-951 bp 1.0 66.2