Hotspot

psbE-petL

LSC · 62023-63245 · 1223 bp

Back to Pseudostellaria

Pi 0.0118

MarkerSeek score 55.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 39
pi: 0.0118
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAATAACTAGTTAGAGTAGGCAT	50.0	30.4	57.8	52.4	1273-1286 bp	1.0	42.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AAATAACTAGTTAGAGTAGGCAT	55.0	30.4	58.2	52.4	1268-1281 bp	1.0	42.9
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAATAACTAGTTAGAGTAGGCAT	50.0	30.4	57.9	52.4	1350-1363 bp	1.0	42.8
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AAATAACTAGTTAGAGTAGGCAT	45.0	30.4	58.2	52.4	1359-1372 bp	1.0	42.8
psbE-petL_p5	CGAATAACCAACCCGCAATG	AAATAACTAGTTAGAGTAGGCAT	50.0	30.4	57.8	52.4	1358-1371 bp	1.0	42.8