Hotspot

petA-psbJ

LSC · 60424-61227 · 804 bp

Back to Pseudostellaria

Pi 0.0169
MarkerSeek score 54.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
804
variable_sites
29
pi
0.0169
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGTCCGA ATTCTGGATTGGGCTCATCG 45.0 50.0 55.4 57.7 652-904 bp 1.0 64.0
petA-psbJ_p2 GAGAAGGTTCAATTGTCCGAA ATTCTGGATTGGGCTCATCG 42.9 50.0 56.2 57.7 652-904 bp 1.0 62.3
petA-psbJ_p3 GAGAAGGTTCAATTGTCCGAAA ATTCTGGATTGGGCTCATCG 40.9 50.0 56.9 57.7 652-904 bp 1.0 60.1
petA-psbJ_p4 AGAAGGTTCAATTGTCCGAA ATTCTGGATTGGGCTCATCG 40.0 50.0 54.9 57.7 651-903 bp 1.0 59.9
petA-psbJ_p5 AGGTTCAATTGTCCGAAATGA ATTCTGGATTGGGCTCATCG 38.1 50.0 55.8 57.7 648-900 bp 1.0 59.1